Arbovirus surveillance of wild-caught mosquitoes is an affordable and sensitive means of monitoring virus transmission dynamics at various spatial-temporal scales, and emergence and re-emergence during epidemic and interepidemic periods. A variety of molecular diagnostics for arbovirus screening of mosquitoes (known as xeno-monitoring) are available, but most provide limited information about virus diversity. Polymerase chain reaction (PCR)-based screening coupled with RNA sequencing is an increasingly affordable and sensitive pipeline for integrating complete viral genome sequencing into surveillance programs. This enables large-scale, high-throughput arbovirus screening from diverse samples. We collected mosquitoes in CO2-baited light traps from five urban parks in Brisbane from March 2021 to May 2022. Mosquito pools of =200 specimens were screened for alphaviruses and flaviviruses using virus genus-specific primers and reverse transcription quantitative PCR (qRT-PCR). A subset of vi...
| Authors | Vieira, CJSP; Onn, MB; Shivas, MA; Shearman, D; Darbro, JM; Graham, M; Freitas, L; van den Hurk, AF; Frentiu, FD; Wallau, GL; Devine, GJ |
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| Journal | Virus evolution |
| Pages | 0 |
| Volume | 10 |
| Date | 25/12/2024 |
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| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1093/ve/veae103 |