Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that causes often debilitating rheumatic disease in tropical Central and South America. There are currently no licensed vaccines or antiviral drugs available for MAYV disease. Here, we generated Mayaro virus-like particles (VLPs) using the scalable baculovirus-insect cell expression system. High-level secretion of MAYV VLPs in the culture fluid of Sf9 insect cells was achieved, and particles with a diameter of 64 to 70?nm were obtained after purification. We characterize a C57BL/6J adult wild-type mouse model of MAYV infection and disease and used this model to compare the immunogenicity of VLPs from insect cells with that of VLPs produced in mammalian cells. Mice received two intramuscular immunizations with 1?µg of nonadjuvanted MAYV VLPs. Potent neutralizing antibody responses were generated against the vaccine strain, BeH407, with comparable activity seen against a contemporary 2018 isolate from Brazil (BR-18), whereas neutra...
| Authors | Abbo, SR; Nguyen, W; Abma-Henkens, MHC; van de Kamer, D; Savelkoul, NHA; Geertsema, C; Le, TTT; Tang, B; Yan, K; Dumenil, T; van Oers, MM; Suhrbier, A; Pijlman, GP |
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| Journal | JOURNAL OF VIROLOGY |
| Pages | e0160122 |
| Volume | 97 |
| Date | 8/04/2023 |
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| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1128/jvi.01601-22 |