RT-qPCR remains a key diagnostic methodology for COVID-19/SARS-CoV-2. Typically, nasal or saliva swabs from patients are placed in virus transport media (VTM), RNA is extracted at the pathology laboratory, and viral RNA is measured using RT-qPCR. In this study, we describe the use of TNA-Cifer Reagent E in a pre-clinical evaluation study to inactivate SARS-CoV-2 as well as prepare samples for RT-qPCR. Adding 1 part TNA-Cifer Reagent E to 5 parts medium containing SARS-CoV-2 for 10 min at room temperature inactivated the virus and permitted RT-qPCR detection. TNA-Cifer Reagent E was compared with established column-based RNA extraction and purification methodology using a panel of human clinical nasal swab samples (n = 61), with TNA-Cifer Reagent E showing high specificity (100%) and sensitivity (97.37%). Mixtures of SARS-CoV-2 virus and TNA-Cifer Reagent E could be stored for 3 days at room temperature or for 2 weeks at 4°C without the loss of RT-qPCR detection sensitivity. The detecti...
| Authors | Pollak, NM; Rawle, DJ; Yan, K; Buckley, C; Le, TT; Wang, CYT; Ertl, NG; van Huyssteen, K; Crkvencic, N; Hashmi, M; Lyons, RE; Whiley, DM; Suhrbier, A; Macdonald, J |
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| Journal | Frontiers in microbiology |
| Pages | 1238542 |
| Volume | 14 |
| Date | 6/11/2023 |
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| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.3389/fmicb.2023.1238542 |