Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots.
Abstract
Zoonotic P. knowlesi and P. cynomolgi symptomatic and asymptomatic infections occur across endemic areas of Southeast Asia. Most infections are low-parasitemia, with an unknown proportion below routine microscopy detection thresholds. Molecular surveillance tools optimizing the limit of detection (LOD) would allow more accurate estimates of zoonotic malaria prevalence.
| Authors | Braima, KA; Piera, KA; Lubis, IND; Noviyanti, R; Rajahram, GS; Kariodimedjo, P; Nainggolan, IRA; Permatasari, R; Trianty, L; Amalia, R; Sakam, SS; Tan, AF; William, T; Westaway, JAF; Lee, P; Daim, S; Surendra, H; Christy, N; Letizia, AG; Peatey, CL; Moideen, MA; Barber, BE; Sutherland, CJ; Anstey, NM; Grigg, MJ |
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| Journal | PLOS NEGLECTED TROPICAL DISEASES |
| Pages | e0012129 |
| Volume | 19 |
| Date | 6/03/2025 |
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| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1371/journal.pntd.0012129 |