Abstract

Objectives: infection and in clinical malaria are poorly understood. Methods: blood stages and in children and adults with acute malaria. Monocyte gene expression and functional phenotypes were examined by RNA sequencing and flow cytometry at peak infection and compared to pre-infection or at convalescence in acute malaria. Results: subsets. In acute malaria, monocyte CD64/Fc?RI expression was significantly increased in children and adults, while HLA-DR remained stable. Although children and adults showed a similar pattern of differentially expressed genes, the number and magnitude of gene expression change were greater in children. Conclusions: Monocyte activation during subpatent malaria is driven by an IFN molecular signature with robust activation of genes enriched in pathogen detection, phagocytosis, antimicrobial activity and antigen presentation. The greater magnitude of transcriptional changes in children with acute malaria suggests monocyte phenotypes may change with age or exposure.

Authors	Loughland, Jessica R; Woodberry, Tonia; Field, Matt; Andrew, Dean W; SheelaNair, Arya; Dooley, Nicholas L; Piera, Kim A; Amante, Fiona H; Kenangalem, Enny; Price, Ric N; Engwerda, Christian R; Anstey, Nicholas M; McCarthy, James S; Boyle, Michelle J; Minigo, Gabriela
Journal	Clinical & translational immunology
Pages	e1144
Volume	9
Date	1/06/2020
Grant ID	1132975
Funding Body	National Health and Medical Research Council (NHMRC)
URL	http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1002/cti2.1144