isolate from Malaysia. K-mer mining of the >40,000 terabases of sequence data in the Sequence Read Archive followed by BLASTn confirmation identified multiple GETV sequences in biosamples from Asia (often as contaminants), but not in biosamples from Australia. In contrast, sequence reads aligning to the Australian Ross River virus (RRV) were readily identified in Australian biosamples. To explore the serological relationship between GETV and other alphaviruses, an adult wild-type mouse model of GETV was established. High levels of cross-reactivity and cross-protection were evident for convalescent sera from mice infected with GETV or RRV, highlighting the difficulties associated with the interpretation of early serosurveys reporting GETV antibodies in Australian cattle and pigs. The evidence that GETV circulates in Australia is thus not compelling.
Authors | Rawle, Daniel J; Nguyen, Wilson; Dumenil, Troy; Parry, Rhys; Warrilow, David; Tang, Bing; Le, Thuy T; Slonchak, Andrii; Khromykh, Alexander A; Lutzky, Viviana P; Yan, Kexin; Suhrbier, Andreas |
---|---|
Journal | Pathogens (Basel, Switzerland) |
Pages | |
Volume | 9 |
Date | 1/10/2020 |
Grant ID | APP1173880 |
Funding Body | National Health and Medical Research Council of Australia |
URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.3390/pathogens9100848 |