Anti-Interleukin-10 Unleashes Transcriptional Response to Leishmanial Antigens in Visceral Leishmaniasis Patients.
Abstract
Visceral leishmaniasis (VL; Leishmania donovani) cases produce interferon-? and tumour necrosis factor in response to soluble leishmanial antigen (SLA) in whole blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti-IL10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6 and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL10 as a potent immune modulator in VL.
| Authors | Singh, Om Prakash; Syn, Genevieve; Nylén, Susanne; Engwerda, Christian; Sacks, David; Wilson, Mary E; Kumar, Rajiv; Chakravarty, Jaya; Sundar, Shyam; Blackwell, Jenefer M; Fakiola, Michaela |
|---|---|
| Journal | The Journal of infectious diseases |
| Pages | 517-521 |
| Volume | 223 |
| Date | 1/07/2020 |
| Grant ID | P50 AI074321 |
| Funding Body | NIAID NIH HHS |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1093/infdis/jiaa381 |