QIMR Berghofer

The role of cardiac transcription factor NKX2-5 in regulating the human cardiac miRNAome.


reporter line to isolate both cardiac committed mesoderm and cardiomyocytes. We identified 11 miRNAs that were differentially expressed in NKX2-5 -expressing cardiac mesoderm compared to non-cardiac mesoderm. Subsequent profiling revealed that the canonical myogenic miRNAs including MIR1-1, MIR133A1 and MIR208A were enriched in cardiomyocytes. Strikingly, deletion of NKX2-5 did not result in gross changes in the cardiac miRNA profile, either at committed mesoderm or cardiomyocyte stages. Thus, in early human cardiomyocyte commitment and differentiation, the cardiac myogenic miRNA program is predominantly regulated independently of the highly conserved NKX2-5 -dependant gene regulatory network.

Authors Arasaratnam, Deevina; Bell, Katrina M; Sim, Choon Boon; Koutsis, Kathy; Anderson, David J; Qian, Elizabeth L; Stanley, Edouard G; Elefanty, Andrew G; Cheung, Michael M; Oshlack, Alicia; White, Anthony J; Khalil, Charbel Abi; Hudson, James E; Porrello, Enzo R; Elliott, David A
Pages 15928
Volume 9
Date 1/11/2019
Grant ID 1006304
Funding Body NHMRC
URL http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1038/s41598-019-52280-9